DOT urine drug testing

1. Check in

• Present a valid form of identification when requested.
• Provide any required paperwork or testing authorization.
• Follow the collector’s instructions throughout the process.

2. Prepare for collection

• Wash and dry your hands when instructed by the collector.
• Do not wash your hands again until you have provided and handed over your specimen.
• Do not bring personal belongings, bags, coats, or unnecessary items into the restroom. Follow the collector’s directions regarding permitted items.

3. Provide your urine specimen

• You will receive a collection cup from the collector.
• Proceed to the designated restroom and provide your urine specimen.
• A DOT urine collection generally requires at least 45 mL (approximately 1.5 ounces).
• Do not flush the toilet.
• Do not add water or any other substance to the specimen.
• Return the specimen directly to the collector as soon as you finish.

4. Specimen processing

• The collector will check the specimen and complete the required documentation.
• DOT collections require a split specimen, which means the collector will divide the urine into two specimen bottles.
• You may be asked to initial the specimen bottle seals and complete the required paperwork.
• Do not open, handle, or seal the specimen bottles unless specifically instructed by the collector.

Important reminders

• Follow all instructions given by the collector.
• Do not attempt to alter, substitute, dilute, or contaminate the specimen.
• If you are unable to provide enough urine, inform the collector immediately. The collector will explain the applicable procedure.

DOT urine collections are conducted under U.S. DOT regulations — 49 CFR Part 40.

Thank you for your cooperation.